Dedication |
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v | (4) |
Contributors |
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ix | (2) |
Preface |
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xi | |
Introduction |
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1 | (3) |
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Chapter 1 Current Problems and Technical Solutions in Protein Biochemistry |
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4 | (117) |
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4 | (2) |
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II. Early Events in Signaling by Interferons: From Membrane Receptor to Induced Genes |
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6 | (2) |
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III. Detection of Systems Components |
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8 | (9) |
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IV. Identification of Components of Complex Systems |
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17 | (33) |
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V. Analysis of Regulatory Modifications of Components of Complex Systems |
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50 | (26) |
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VI. Analysis of Protein: Ligand Complexes |
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76 | (17) |
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93 | (1) |
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94 | (27) |
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Chapter 2 Posttranslational Modifications |
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121 | (87) |
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121 | (4) |
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II. Types of Posttranslational Derivatives |
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125 | (34) |
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III. Types of Posttranslational Reactions: How Modifications Are Made |
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159 | (16) |
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IV. Biological Functions of Posttranslational Modifications: Why Modifications Are Made |
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175 | (17) |
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192 | (16) |
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Chapter 3 Design and Use of Synthetic Peptides as Biological Models |
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208 | (51) |
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I. Peptides as Structural Models of Proteins |
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208 | (11) |
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II. Cell/Peptide Interactions |
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219 | (9) |
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III. Protein/Peptide Interactions |
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228 | (8) |
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IV. Peptides and Modulation of T-Cell Behavior |
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236 | (4) |
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V. Enzyme/Peptide Interactions: Peptides as Enzyme Substrates |
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240 | (3) |
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243 | (1) |
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243 | (16) |
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Chapter 4 Site-Directed Mutagenesis and Protein Engineering |
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259 | (54) |
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259 | (1) |
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260 | (1) |
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III. Techniques for Oligonucleotide-Directed Mutagenesis |
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261 | (11) |
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IV. Strategies for Engineering Proteins |
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272 | (19) |
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V. Case Studies: Introduction of Function by Site-Directed Mutagenesis |
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291 | (11) |
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302 | (1) |
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303 | (10) |
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Chapter 5 De Novo Design of Protein Structure and Function |
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313 | (42) |
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I. Introduction and Overview |
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313 | (1) |
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II. De Novo Design of Structure |
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314 | (5) |
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III. Characterizing the Design |
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319 | (1) |
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IV. Selected Examples of Designed Proteins |
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320 | (11) |
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V. De Novo Design of Function |
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331 | (13) |
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VI. Design of Catalytic Proteins |
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344 | (4) |
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VII. Summary and Future Directions |
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348 | (1) |
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348 | (7) |
Index |
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355 | |